How To Convert your drives from FAT to NTFS in Windows Vista
If you've upgraded your PC from an earlier version of Windows, there's a chance you're still using the FAT32 filesystem. Assuming you don't need to keep FAT32 for compatibility with other operating systems, you should convert your drive to NTFS. The process is easy, relatively quick, and won't harm your data (although you should back up beforehand just to be safe).